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10.1038/srep11800 http://scihub22266oqcxt.onion/10.1038/srep11800 C4488866!4488866 !26135970     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=26135970 &cmd=llinks): Failed to open stream: HTTP request failed! HTTP/1.1 429 Too Many Requests in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 215 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\26135970 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Sci+Rep 2015 ; 5 (ä): 11800 Nephropedia Template TP {{tp|p=26135970 |t=2015. Controlled electromechanical cell stimulation on-a-chip |pdf=|usr=}}{{26135970 }} gab.com Text Controlled electromechanical cell stimulation on-a-chip JO: Sci Rep http://www.kidney.de/pget.php?&tw=1&pmid=26135970 #FOAvip Stem cell research has yielded promising advances in regenerative medicine, but standard assays generally lack the ability to combine different cell stimulations with rapid sample processing and precise fluid control. In this work, we describe the design and fabrication of a micro-scale cell stimulator capable of simultaneously providing mechanical, electrical, and biochemical stimulation, and subsequently extracting detailed morphological and gene-expression analysis on the cellular response. This micro-device offers the opportunity to overcome previous limitations and recreate critical elements of the in vivo microenvironment in order to investigate cellular responses to three different stimulations. The platform was validated in experiments using human bone marrow mesenchymal stem cells. These experiments demonstrated the ability for inducing changes in cell morphology, cytoskeletal fiber orientation and changes in gene expression under physiological stimuli. This novel bioengineering approach can be readily applied to various studies, especially in the fields of stem cell biology and regenerative medicine. Twit Text FOAVip Controlled electromechanical cell stimulation on-a-chip ????Sci Rep http://www.kidney.de/pget.php?&tw=1&pmid=26135970 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=26135970 #FOAvip English Wikipedia <ref>{{Cite pmid|26135970 }}</ref> Controlled electromechanical cell stimulation on-a-chip #MMPMID26135970 Pavesi A ; Adriani G ; Rasponi M ; Zervantonakis IK ; Fiore GB ; Kamm RD Sci Rep 2015[Jul]; 5 (ä): 11800 PMID26135970 show ga Stem cell research has yielded promising advances in regenerative medicine, but standard assays generally lack the ability to combine different cell stimulations with rapid sample processing and precise fluid control. In this work, we describe the design and fabrication of a micro-scale cell stimulator capable of simultaneously providing mechanical, electrical, and biochemical stimulation, and subsequently extracting detailed morphological and gene-expression analysis on the cellular response. This micro-device offers the opportunity to overcome previous limitations and recreate critical elements of the in vivo microenvironment in order to investigate cellular responses to three different stimulations. The platform was validated in experiments using human bone marrow mesenchymal stem cells. These experiments demonstrated the ability for inducing changes in cell morphology, cytoskeletal fiber orientation and changes in gene expression under physiological stimuli. This novel bioengineering approach can be readily applied to various studies, especially in the fields of stem cell biology and regenerative medicine. |*Cell Differentiation [MESH] |*Regenerative Medicine [MESH] |Bone Marrow Cells/cytology/metabolism [MESH] |Electric Stimulation [MESH] |Gene Expression [MESH] |Humans [MESH] |Mesenchymal Stem Cells/*cytology/metabolism [MESH]Controlled electromechanical cell stimulation on-a-chip (epmc).pdf DeepDyve Pubget Overpricing