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2015 ; 4
(ä): 127-9
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Contribution of nuclear actin to transcription regulation
#MMPMID26484196
Yamazaki S
; Yamamoto K
; Harata M
Genom Data
2015[Jun]; 4
(ä): 127-9
PMID26484196
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Actin, an integral component of the cytoskeleton, plays crucial roles in a
variety of cell functions, including cell migration, adhesion, polarity and shape
change. Studies performed during the last couple of decades have revealed that
the actin also exists in the nucleus. However, the function and properties of
nuclear actin remained elusive so far. Recently, we showed that an actin tagged
with EYFP and fused with a nuclear localization signal (EYFP-NLS-actin) formed
visible filamentous (F)-actin bundles in cells. To obtain further details about
the individual genes that are affected by the nuclear actin, we have used the
microarray analysis to determine the changes in the expression levels of RNAs in
HeLa cells as a result of EYFP-NLS-actin expression. Our results suggest that the
nuclear actin plays a role in the activation of genes rather than their
repression. The data has been deposited in the Gene Expression Omnibus (GEO)
database under the accession number GSE59799.