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.jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117 Nat+Commun
2015 ; 6
(ä): 8881
Nephropedia Template TP
de Medeiros G
; Norlin N
; Gunther S
; Albert M
; Panavaite L
; Fiuza UM
; Peri F
; Hiiragi T
; Krzic U
; Hufnagel L
Nat Commun
2015[Nov]; 6
(ä): 8881
PMID26602977
show ga
Selective-plane illumination microscopy has proven to be a powerful imaging
technique due to its unsurpassed acquisition speed and gentle optical sectioning.
However, even in the case of multiview imaging techniques that illuminate and
image the sample from multiple directions, light scattering inside tissues often
severely impairs image contrast. Here we combine multiview light-sheet imaging
with electronic confocal slit detection implemented on modern camera sensors. In
addition to improved imaging quality, the electronic confocal slit detection
doubles the acquisition speed in multiview setups with two opposing illumination
directions allowing simultaneous dual-sided illumination. Confocal multiview
light-sheet microscopy eliminates the need for specimen-specific data fusion
algorithms, streamlines image post-processing, easing data handling and storage.