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2018 ; 10
(4
): ä Nephropedia Template TP
gab.com Text
Twit Text FOAVip
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English Wikipedia
Concepts in Light Microscopy of Viruses
#MMPMID29670029
Witte R
; Andriasyan V
; Georgi F
; Yakimovich A
; Greber UF
Viruses
2018[Apr]; 10
(4
): ä PMID29670029
show ga
Viruses threaten humans, livestock, and plants, and are difficult to combat.
Imaging of viruses by light microscopy is key to uncover the nature of known and
emerging viruses in the quest for finding new ways to treat viral disease and
deepening the understanding of virus–host interactions. Here, we provide an
overview of recent technology for imaging cells and viruses by light microscopy,
in particular fluorescence microscopy in static and live-cell modes. The review
lays out guidelines for how novel fluorescent chemical probes and proteins can be
used in light microscopy to illuminate cells, and how they can be used to study
virus infections. We discuss advantages and opportunities of confocal and
multi-photon microscopy, selective plane illumination microscopy, and
super-resolution microscopy. We emphasize the prevalent concepts in image
processing and data analyses, and provide an outlook into label-free digital
holographic microscopy for virus research.