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10.1002/dvdy.24149 http://scihub22266oqcxt.onion/10.1002/dvdy.24149 C4140996!4140996 !24866848     free     free     free Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\24866848 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Dev+Dyn 2014 ; 243 (9 ): 1095-105 Nephropedia Template TP {{tp|p=24866848 |t=2014. Concentration-dependent effects of WNTLESS on WNT1/3A signaling |pdf=|usr=}}{{24866848 }} gab.com Text Concentration-dependent effects of WNTLESS on WNT1/3A signaling JO: Dev Dyn http://www.kidney.de/pget.php?&tw=1&pmid=24866848 #FOAvip BACKGROUND: WNTLESS (WLS) is a multi-transmembrane protein that transports Wnt ligands from the Golgi to the cell surface. Although WLS loss-of-function experiments in the developing central nervous system reveal phenotypes consistent with defects in WNT1 and WNT3A signaling, data from complementary gain-of-function experiments have not yet been reported. Here, we report the phenotypic consequences of WLS overexpression in cultured cells and in the developing chick spinal cord. RESULTS: Overexpression of small amounts of WLS along with either WNT1 or WNT3A promotes the Wnt/?-catenin pathway in HEK293T cells, while overexpression of higher levels of WLS inhibits the Wnt/?-catenin pathway in these cells. Similarly, overexpressed WLS inhibits the Wnt/?-catenin pathway in the developing spinal cord, as assessed by cell proliferation and specification. These effects appear to be Wnt-specific as overexpression of WLS inhibits the expression of FZD10, a target of ?-catenin-dependent transcription. CONCLUSIONS: Our results show that overexpression of WLS inhibits Wnt/?-catenin signaling in the spinal cord. As the activation of the Wnt/?-catenin pathway in the spinal cord requires WNT1 or WNT3A, our results are consistent with a model in which the relative concentration of WLS to Wnt regulates WNT1/3A signaling in the developing spinal cord. Twit Text FOAVip Concentration-dependent effects of WNTLESS on WNT1/3A signaling ????Dev Dyn http://www.kidney.de/pget.php?&tw=1&pmid=24866848 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=24866848 #FOAvip English Wikipedia <ref>{{Cite pmid|24866848 }}</ref> Concentration-dependent effects of WNTLESS on WNT1/3A signaling #MMPMID24866848 Galli LM ; Szabo LA ; Li L ; Htaik YM ; Onguka O ; Burrus LW Dev Dyn 2014[Sep]; 243 (9 ): 1095-105 PMID24866848 show ga BACKGROUND: WNTLESS (WLS) is a multi-transmembrane protein that transports Wnt ligands from the Golgi to the cell surface. Although WLS loss-of-function experiments in the developing central nervous system reveal phenotypes consistent with defects in WNT1 and WNT3A signaling, data from complementary gain-of-function experiments have not yet been reported. Here, we report the phenotypic consequences of WLS overexpression in cultured cells and in the developing chick spinal cord. RESULTS: Overexpression of small amounts of WLS along with either WNT1 or WNT3A promotes the Wnt/?-catenin pathway in HEK293T cells, while overexpression of higher levels of WLS inhibits the Wnt/?-catenin pathway in these cells. Similarly, overexpressed WLS inhibits the Wnt/?-catenin pathway in the developing spinal cord, as assessed by cell proliferation and specification. These effects appear to be Wnt-specific as overexpression of WLS inhibits the expression of FZD10, a target of ?-catenin-dependent transcription. CONCLUSIONS: Our results show that overexpression of WLS inhibits Wnt/?-catenin signaling in the spinal cord. As the activation of the Wnt/?-catenin pathway in the spinal cord requires WNT1 or WNT3A, our results are consistent with a model in which the relative concentration of WLS to Wnt regulates WNT1/3A signaling in the developing spinal cord. |Animals [MESH] |Cell Proliferation [MESH] |Chick Embryo [MESH] |HEK293 Cells [MESH] |Humans [MESH] |Intracellular Signaling Peptides and Proteins/*genetics/metabolism [MESH] |Neurons/metabolism [MESH] |Receptors, G-Protein-Coupled/*genetics/metabolism [MESH] |Spinal Cord/metabolism [MESH] |Wnt Proteins/*metabolism [MESH]Concentration-dependent effects of WNTLESS on WNT1/3A signaling (epmc).pdf DeepDyve Pubget Overpricing