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2016 ; 6
(ä): 27614
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Compressed Sensing Electron Tomography for Determining Biological Structure
#MMPMID27291259
Guay MD
; Czaja W
; Aronova MA
; Leapman RD
Sci Rep
2016[Jun]; 6
(ä): 27614
PMID27291259
show ga
There has been growing interest in applying compressed sensing (CS) theory and
practice to reconstruct 3D volumes at the nanoscale from electron tomography
datasets of inorganic materials, based on known sparsity in the structure of
interest. Here we explore the application of CS for visualizing the 3D structure
of biological specimens from tomographic tilt series acquired in the scanning
transmission electron microscope (STEM). CS-ET reconstructions match or
outperform commonly used alternative methods in full and undersampled tomogram
recovery, but with less significant performance gains than observed for the
imaging of inorganic materials. We propose that this disparity stems from the
increased structural complexity of biological systems, as supported by
theoretical CS sampling considerations and numerical results in simulated phantom
datasets. A detailed analysis of the efficacy of CS-ET for undersampled recovery
is therefore complicated by the structure of the object being imaged. The
numerical nonlinear decoding process of CS shares strong connections with popular
regularized least-squares methods, and the use of such numerical recovery
techniques for mitigating artifacts and denoising in reconstructions of fully
sampled datasets remains advantageous. This article provides a link to the
software that has been developed for CS-ET reconstruction of electron tomographic
data sets.