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2016 ; 166
(3
): 651-663
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Compositional Control of Phase-Separated Cellular Bodies
#MMPMID27374333
Banani SF
; Rice AM
; Peeples WB
; Lin Y
; Jain S
; Parker R
; Rosen MK
Cell
2016[Jul]; 166
(3
): 651-663
PMID27374333
show ga
Cellular bodies such as P bodies and PML nuclear bodies (PML NBs) appear to be
phase-separated liquids organized by multivalent interactions among proteins and
RNA molecules. Although many components of various cellular bodies are known,
general principles that define body composition are lacking. We modeled cellular
bodies using several engineered multivalent proteins and RNA. In vitro and in
cells, these scaffold molecules form phase-separated liquids that concentrate low
valency client proteins. Clients partition differently depending on the ratio of
scaffolds, with a sharp switch across the phase diagram diagonal. Composition can
switch rapidly through changes in scaffold concentration or valency. Natural PML
NBs and P bodies show analogous partitioning behavior, suggesting how their
compositions could be controlled by levels of PML SUMOylation or cellular mRNA
concentration, respectively. The data suggest a conceptual framework for
considering the composition and control thereof of cellular bodies assembled
through heterotypic multivalent interactions.
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