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2014 ; 86
(21
): 10520-3
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Collection of analytes from microneedle patches
#MMPMID25367229
Romanyuk AV
; Zvezdin VN
; Samant P
; Grenader MI
; Zemlyanova M
; Prausnitz MR
Anal Chem
2014[Nov]; 86
(21
): 10520-3
PMID25367229
show ga
Clinical medicine and public health would benefit from simplified acquisition of
biological samples from patients that can be easily obtained at point of care, in
the field, and by patients themselves. Microneedle patches are designed to serve
this need by collecting dermal interstitial fluid containing biomarkers without
the dangers, pain, or expertise needed to collect blood. This study presents
novel methods to collect biomarker analytes from microneedle patches for analysis
by integration into conventional analytical laboratory microtubes and
microplates. Microneedle patches were made out of cross-linked hydrogel composed
of poly(methyl vinyl ether-alt-maleic acid) and poly(ethylene glycol) prepared by
micromolding. Microneedle patches were shown to swell with water up to 50-fold in
volume, depending on degree of polymer cross-linking, and to collect interstitial
fluid from the skin of rats. To collect analytes from microneedle patches, the
patches were mounted within the cap of microcentrifuge tubes or formed the top of
V-bottom multiwell microplates, and fluid was collected in the bottom of the
tubes under gentle centrifugation. In another method, microneedle patches were
attached to form the bottom of multiwell microplates, thereby enabling in situ
analysis. The simplicity of biological sample acquisition using microneedle
patches coupled with the simplicity of analyte collection from microneedles
patches integrated into conventional analytical equipment could broaden the reach
of future screening, diagnosis, and monitoring of biomarkers in healthcare and
environmental/workplace settings.
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