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2014 ; 35
(7
): 937-55
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Clinical applications of capillary electrophoresis based immunoassays
#MMPMID24132682
Moser AC
; Willicott CW
; Hage DS
Electrophoresis
2014[Apr]; 35
(7
): 937-55
PMID24132682
show ga
Immunoassays have long been an important set of tools in clinical laboratories
for the detection, diagnosis, and treatment of disease. Over the last two
decades, there has been growing interest in utilizing CE as a means for
conducting immunoassays with clinical samples. The resulting method is known as a
CE immunoassay. This approach makes use of the selective and strong binding of
antibodies for their targets, as is employed in a traditional immunoassay, and
combines this with the speed, efficiency, and small sample requirements of CE.
This review discusses the variety of ways in which CE immunoassays have been
employed with clinical samples. An overview of the formats and detection modes
that have been employed in these applications is first presented. A more detailed
discussion is then given on the type of clinical targets and samples that have
been measured or studied by using CE immunoassays. Particular attention is given
to the use of this method in the fields of endocrinology, pharmaceutical
measurements, protein and peptide analysis, immunology, infectious disease
detection, and oncology. Representative applications in each of these areas are
described, with these examples involving work with both traditional and
microanalytical CE systems.