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2015 ; 552
(ä): 165-84
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Circadian regulation of cellular physiology
#MMPMID25707277
Peek CB
; Ramsey KM
; Levine DC
; Marcheva B
; Perelis M
; Bass J
Methods Enzymol
2015[]; 552
(ä): 165-84
PMID25707277
show ga
The circadian clock synchronizes behavioral and physiological processes on a
daily basis in anticipation of the light-dark cycle. In mammals, molecular clocks
are present in both the central pacemaker neurons and in nearly all peripheral
tissues. Clock transcription factors in metabolic tissues coordinate metabolic
fuel utilization and storage with alternating periods of feeding and fasting
corresponding to the rest-activity cycle. In vitro and in vivo biochemical
approaches have led to the discovery of mechanisms underlying the interplay
between the molecular clock and the metabolic networks. For example, recent
studies have demonstrated that the circadian clock controls rhythmic synthesis of
the cofactor nicotinamide adenine dinucleotide (NAD(+)) and activity of
NAD(+)-dependent sirtuin deacetylase enzymes to regulate mitochondrial function
across the circadian cycle. In this chapter, we review current state-of-the-art
methods to analyze circadian cycles in mitochondrial bioenergetics, glycolysis,
and nucleotide metabolism in both cell-based and animal models.