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10.1002/dvg.22915 http://scihub22266oqcxt.onion/10.1002/dvg.22915 C4819711!4819711 !26742453     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=26742453 &cmd=llinks): Failed to open stream: HTTP request failed! HTTP/1.1 429 Too Many Requests in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 215 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\26742453 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Genesis 2016 ; 54 (2 ): 78-85 Nephropedia Template TP {{tp|p=26742453 |t=2016. Chromosome engineering in zygotes with CRISPR/Cas9 |pdf=|usr=}}{{26742453 }} gab.com Text Chromosome engineering in zygotes with CRISPR/Cas9 JO: Genesis http://www.kidney.de/pget.php?&tw=1&pmid=26742453 #FOAvip Deletions, duplications, and inversions of large genomic regions covering several genes are an important class of disease causing variants in humans. Modeling these structural variants in mice requires multistep processes in ES cells, which has limited their availability. Mutant mice containing small insertions, deletions, and single nucleotide polymorphisms can be reliably generated using CRISPR/Cas9 directly in mouse zygotes. Large structural variants can be generated using CRISPR/Cas9 in ES cells, but it has not been possible to generate these directly in zygotes. We now demonstrate the direct generation of deletions, duplications and inversions of up to one million base pairs by zygote injection. Twit Text FOAVip Chromosome engineering in zygotes with CRISPR/Cas9 ????Genesis http://www.kidney.de/pget.php?&tw=1&pmid=26742453 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=26742453 #FOAvip English Wikipedia <ref>{{Cite pmid|26742453 }}</ref> Chromosome engineering in zygotes with CRISPR/Cas9 #MMPMID26742453 Boroviak K ; Doe B ; Banerjee R ; Yang F ; Bradley A Genesis 2016[Feb]; 54 (2 ): 78-85 PMID26742453 show ga Deletions, duplications, and inversions of large genomic regions covering several genes are an important class of disease causing variants in humans. Modeling these structural variants in mice requires multistep processes in ES cells, which has limited their availability. Mutant mice containing small insertions, deletions, and single nucleotide polymorphisms can be reliably generated using CRISPR/Cas9 directly in mouse zygotes. Large structural variants can be generated using CRISPR/Cas9 in ES cells, but it has not been possible to generate these directly in zygotes. We now demonstrate the direct generation of deletions, duplications and inversions of up to one million base pairs by zygote injection. |*CRISPR-Cas Systems [MESH] |*Chromosomes [MESH] |Animals [MESH] |Base Sequence [MESH] |Chromosome Duplication [MESH] |Chromosome Inversion [MESH] |DNA [MESH] |Feasibility Studies [MESH] |Female [MESH] |Genetic Engineering/*methods [MESH] |Male [MESH] |Mice [MESH] |Mice, Inbred C57BL [MESH]Chromosome engineering in zygotes with CRISPR/Cas9 (epmc).pdf DeepDyve Pubget Overpricing