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2016 ; 26
(9
): 1245-56
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Chromatin dynamics during DNA replication
#MMPMID27225843
Bar-Ziv R
; Voichek Y
; Barkai N
Genome Res
2016[Sep]; 26
(9
): 1245-56
PMID27225843
show ga
Chromatin is composed of DNA and histones, which provide a unified platform for
regulating DNA-related processes, mostly through their post-translational
modification. During DNA replication, histone arrangement is perturbed, first to
allow progression of DNA polymerase and then during repackaging of the replicated
DNA. To study how DNA replication influences the pattern of histone modification,
we followed the cell-cycle dynamics of 10 histone marks in budding yeast. We find
that histones deposited on newly replicated DNA are modified at different rates:
While some marks appear immediately upon replication (e.g., H4K16ac, H3K4me1),
others increase with transcription-dependent delays (e.g., H3K4me3, H3K36me3).
Notably, H3K9ac was deposited as a wave preceding the replication fork by ?5-6
kb. This replication-guided H3K9ac was fully dependent on the acetyltransferase
Rtt109, while expression-guided H3K9ac was deposited by Gcn5. Further,
topoisomerase depletion intensified H3K9ac in front of the replication fork and
in sites where RNA polymerase II was trapped, suggesting supercoiling stresses
trigger H3K9 acetylation. Our results assign complementary roles for DNA
replication and gene expression in defining the pattern of histone modification.
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