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2015 ; 99
(Pt B
): 189-93
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Cholesterol metabolites exported from human brain
#MMPMID25668615
Iuliano L
; Crick PJ
; Zerbinati C
; Tritapepe L
; Abdel-Khalik J
; Poirot M
; Wang Y
; Griffiths WJ
Steroids
2015[Jul]; 99
(Pt B
): 189-93
PMID25668615
show ga
The human brain contains approximately 25% of the body's cholesterol. The brain
is separated from the circulation by the blood brain barrier. While cholesterol
will not passes this barrier, oxygenated forms of cholesterol can cross the
barrier. Here by measuring the difference in the oxysterol content of blood
plasma in the jugular vein and in a forearm vein by mass spectrometry (MS) we
were able to determine the flux of more than 20 cholesterol metabolites between
brain and the circulation. We confirm that 24S-hydroxycholesterol is exported
from brain at a rate of about 2-3mg/24h. Gas chromatography (GC)-MS data shows
that the cholesterol metabolites 5?-hydroxy-6-oxocholesterol
(3?,5?-dihydroxycholestan-6-one), 7?-hydroxycholesterol and 7-oxocholesterol,
generally considered to be formed through reactive oxygen species, are similarly
exported from brain at rates of about 0.1, 2 and 2mg/24h, respectively. Although
not to statistical significance both GC-MS and liquid chromatography (LC)-MS
methods indicate that (25R)26-hydroxycholesterol is imported to brain, while
LC-MS indicates that 7?-hydroxy-3-oxocholest-4-enoic acid is exported from brain.
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