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2016 ; 90
(20
): 8950-3
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Characterization of Viral Populations by Using Circular Sequencing
#MMPMID27440878
Whitfield ZJ
; Andino R
J Virol
2016[Oct]; 90
(20
): 8950-3
PMID27440878
show ga
With the enormous sizes viral populations reach, many variants are at too low a
frequency to be detected by conventional next-generation sequencing (NGS)
methods. Circular sequencing (CirSeq) is a method by which the error rate of
next-generation sequencing is decreased so that even low-frequency viral variants
can be accurately detected. The ability to visualize almost the entire genetic
makeup of a viral swarm has implications for epidemiology, viral evolution, and
vaccine design. Here we discuss experimental planning, analysis, and recent
insights using CirSeq.