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2011 ; 106
(ä): 325-41
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Cell identification and cell lineage analysis
#MMPMID22118283
Giurumescu CA
; Chisholm AD
Methods Cell Biol
2011[]; 106
(ä): 325-41
PMID22118283
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Caenorhabditis elegans is uniquely suited to the analysis of cell lineage
patterns. C. elegans has a small number of somatic cells whose position and
morphology are almost invariant from animal to animal. Because C. elegans is
virtually transparent, cells can be identified in live animals using a simple
bright-field microscopy technique, Nomarski differential interference contrast
(DIC), or by expression of transgenic fluorescent reporter genes. The small size
and rapid development of C. elegans mean that animals can develop while under
continuous observation, allowing cell lineages to be analyzed throughout
embryonic and postembryonic development. Embryonic cell lineages can also be
traced semiautomatically using timelapse imaging of GFP-labeled nuclei. Analysis
of mutant cell lineages remains important for defining the roles of developmental
control genes.