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2006 ; ä (ä): 1-40
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Cell division
#MMPMID18050484
Oegema K
; Hyman AA
WormBook
2006[Jan]; ä (ä): 1-40
PMID18050484
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The C. elegans embryo is a powerful model system for studying the mechanics of
metazoan cell division. Its primary advantage is that the architecture of the
syncytial gonad makes it possible to use RNAi to generate oocytes whose cytoplasm
is reproducibly (typically >95%) depleted of targeted essential gene products via
a process that does not depend exclusively on intrinsic protein turnover. The
depleted oocytes can then be analyzed as they attempt their first mitotic
division following fertilization. Here we outline the characteristics that
contribute to the usefulness of the C. elegans embryo for cell division studies.
We provide a timeline for the first embryonic mitosis and highlight some of its
key features. We also summarize some of the recent discoveries made using this
system, particularly in the areas of nuclear envelope assembly/dissassembly,
centrosome dynamics, formation of the mitotic spindle, kinetochore assembly,
chromosome segregation, and cytokinesis.