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2015 ; 7
(3
): a016535
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Cell biology of mitotic recombination
#MMPMID25731763
Lisby M
; Rothstein R
Cold Spring Harb Perspect Biol
2015[Mar]; 7
(3
): a016535
PMID25731763
show ga
Homologous recombination provides high-fidelity DNA repair throughout all domains
of life. Live cell fluorescence microscopy offers the opportunity to image
individual recombination events in real time providing insight into the in vivo
biochemistry of the involved proteins and DNA molecules as well as the cellular
organization of the process of homologous recombination. Herein we review the
cell biological aspects of mitotic homologous recombination with a focus on
Saccharomyces cerevisiae and mammalian cells, but will also draw on findings from
other experimental systems. Key topics of this review include the stoichiometry
and dynamics of recombination complexes in vivo, the choreography of assembly and
disassembly of recombination proteins at sites of DNA damage, the mobilization of
damaged DNA during homology search, and the functional compartmentalization of
the nucleus with respect to capacity of homologous recombination.