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2015 ; 5
(ä): 11835
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Carbon Quantum Dots for Zebrafish Fluorescence Imaging
#MMPMID26135470
Kang YF
; Li YH
; Fang YW
; Xu Y
; Wei XM
; Yin XB
Sci Rep
2015[Jul]; 5
(ä): 11835
PMID26135470
show ga
Carbon quantum dots (C-QDs) are becoming a desirable alternative to metal-based
QDs and dye probes owing to their high biocompatibility, low toxicity, ease of
preparation, and unique photophysical properties. Herein, we describe
fluorescence bioimaging of zebrafish using C-QDs as probe in terms of the
preparation of C-QDs, zebrafish husbandry, embryo harvesting, and introduction of
C-QDs into embryos and larvae by soaking and microinjection. The multicolor of
C-QDs was validated with their imaging for zebrafish embryo. The distribution of
C-QDs in zebrafish embryos and larvae were successfully observed from their
fluorescence emission. the bio-toxicity of C-QDs was tested with zebrafish as
model and C-QDs do not interfere to the development of zebrafish embryo. All of
the results confirmed the high biocompatibility and low toxicity of C-QDs as
imaging probe. The absorption, distribution, metabolism and excretion route
(ADME) of C-QDs in zebrafish was revealed by their distribution. Our work
provides the useful information for the researchers interested in studying with
zebrafish as a model and the applications of C-QDs. The operations related
zebrafish are suitable for the study of the toxicity, adverse effects, transport,
and biocompatibility of nanomaterials as well as for drug screening with
zebrafish as model.