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2017 ; 7
(1
): 9269
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CUBIC pathology: three-dimensional imaging for pathological diagnosis
#MMPMID28839164
Nojima S
; Susaki EA
; Yoshida K
; Takemoto H
; Tsujimura N
; Iijima S
; Takachi K
; Nakahara Y
; Tahara S
; Ohshima K
; Kurashige M
; Hori Y
; Wada N
; Ikeda JI
; Kumanogoh A
; Morii E
; Ueda HR
Sci Rep
2017[Aug]; 7
(1
): 9269
PMID28839164
show ga
The examination of hematoxylin and eosin (H&E)-stained tissues on glass slides by
conventional light microscopy is the foundation for histopathological diagnosis.
However, this conventional method has some limitations in x-y axes due to its
relatively narrow range of observation area and in z-axis due to its
two-dimensionality. In this study, we applied a CUBIC pipeline, which is the most
powerful tissue-clearing and three-dimensional (3D)-imaging technique, to
clinical pathology. CUBIC was applicable to 3D imaging of both normal and
abnormal patient-derived, human lung and lymph node tissues. Notably, the
combination of deparaffinization and CUBIC enabled 3D imaging of specimens
derived from paraffin-embedded tissue blocks, allowing quantitative evaluation of
nuclear and structural atypia of an archival malignant lymphoma tissue.
Furthermore, to examine whether CUBIC can be applied to practical use in
pathological diagnosis, we performed a histopathological screening of a lymph
node metastasis based on CUBIC, which successfully improved the sensitivity in
detecting minor metastatic carcinoma nodules in lymph nodes. Collectively, our
results indicate that CUBIC significantly contributes to retrospective and
prospective clinicopathological diagnosis, which might lead to the establishment
of a novel field of medical science based on 3D histopathology.