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2018 ; 9
(ä): 240
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CRISPR/Cascade 9-Mediated Genome Editing-Challenges and Opportunities
#MMPMID30026755
Roy B
; Zhao J
; Yang C
; Luo W
; Xiong T
; Li Y
; Fang X
; Gao G
; Singh CO
; Madsen L
; Zhou Y
; Kristiansen K
Front Genet
2018[]; 9
(ä): 240
PMID30026755
show ga
Clustered Regularly Interspaced Palindromic Repeats (CRISPR) and Cascade 9 (also
known as Cas9, CRISPR associated protein 9) confer protection against invading
viruses or plasmids. The CRISPR/Cascade 9 system constitutes one of the most
powerful genome technologies available to researchers today. So far, this
technology has enabled efficient genome editing and modification in several model
organisms and has successfully been used in biomedicine and biomedical
engineering. However, challenges for efficient and safe genetic manipulation in
several organisms persist. Here, we review functional approaches and future
challenges associated with the use of the CRISPR/Cascade 9 genome editing system
and discuss opportunities, ethical issues and future directions within this
field.