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10.15252/msb.20177834 http://scihub22266oqcxt.onion/10.15252/msb.20177834 C5658704!5658704 !28993443     free     free     free Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\28993443 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Mol+Syst+Biol 2017 ; 13 (10 ): 945 Nephropedia Template TP {{tp|p=28993443 |t=2017. CRISPR/Cas9 screening using unique molecular identifiers |pdf=|usr=}}{{28993443 }} gab.com Text CRISPR/Cas9 screening using unique molecular identifiers JO: Mol Syst Biol http://www.kidney.de/pget.php?&tw=1&pmid=28993443 #FOAvip Loss-of-function screening by CRISPR/Cas9 gene knockout with pooled, lentiviral guide libraries is a widely applicable method for systematic identification of genes contributing to diverse cellular phenotypes. Here, Random Sequence Labels (RSLs) are incorporated into the guide library, which act as unique molecular identifiers (UMIs) to allow massively parallel lineage tracing and lineage dropout screening. RSLs greatly improve the reproducibility of results by increasing both the precision and the accuracy of screens. They reduce the number of cells needed to reach a set statistical power, or allow a more robust screen using the same number of cells. Twit Text FOAVip CRISPR/Cas9 screening using unique molecular identifiers ????Mol Syst Biol http://www.kidney.de/pget.php?&tw=1&pmid=28993443 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=28993443 #FOAvip English Wikipedia <ref>{{Cite pmid|28993443 }}</ref> CRISPR/Cas9 screening using unique molecular identifiers #MMPMID28993443 Schmierer B ; Botla SK ; Zhang J ; Turunen M ; Kivioja T ; Taipale J Mol Syst Biol 2017[Oct]; 13 (10 ): 945 PMID28993443 show ga Loss-of-function screening by CRISPR/Cas9 gene knockout with pooled, lentiviral guide libraries is a widely applicable method for systematic identification of genes contributing to diverse cellular phenotypes. Here, Random Sequence Labels (RSLs) are incorporated into the guide library, which act as unique molecular identifiers (UMIs) to allow massively parallel lineage tracing and lineage dropout screening. RSLs greatly improve the reproducibility of results by increasing both the precision and the accuracy of screens. They reduce the number of cells needed to reach a set statistical power, or allow a more robust screen using the same number of cells. |*Gene Knockout Techniques [MESH] |CRISPR-Cas Systems [MESH] |Cell Line [MESH] |Gene Library [MESH] |HEK293 Cells [MESH] |Humans [MESH]CRISPR/Cas9 screening using unique molecular identifiers (epmc).pdf DeepDyve Pubget Overpricing