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10.1186/s12864-018-4713-y http://scihub22266oqcxt.onion/10.1186/s12864-018-4713-y C5930688!5930688 !29720086     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=29720086 &cmd=llinks): Failed to open stream: HTTP request failed! HTTP/1.1 429 Too Many Requests in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 215 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\29720086 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       BMC+Genomics 2018 ; 19 (1 ): 318 Nephropedia Template TP {{tp|p=29720086 |t=2018. CLICK: one-step generation of conditional knockout mice |pdf=|usr=}}{{29720086 }} gab.com Text CLICK: one-step generation of conditional knockout mice JO: BMC Genomics http://www.kidney.de/pget.php?&tw=1&pmid=29720086 #FOAvip BACKGROUND: CRISPR/Cas9 enables the targeting of genes in zygotes; however, efficient approaches to create loxP-flanked (floxed) alleles remain elusive. RESULTS: Here, we show that the electroporation of Cas9, two gRNAs, and long single-stranded DNA (lssDNA) into zygotes, termed CLICK (CRISPR with lssDNA inducing conditional knockout alleles), enables the quick generation of floxed alleles in mice and rats. CONCLUSIONS: The high efficiency of CLICK provides homozygous knock-ins in oocytes carrying tissue-specific Cre, which allows the one-step generation of conditional knockouts in founder (F0) mice. Twit Text FOAVip CLICK: one-step generation of conditional knockout mice ????BMC Genomics http://www.kidney.de/pget.php?&tw=1&pmid=29720086 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=29720086 #FOAvip English Wikipedia <ref>{{Cite pmid|29720086 }}</ref> CLICK: one-step generation of conditional knockout mice #MMPMID29720086 Miyasaka Y ; Uno Y ; Yoshimi K ; Kunihiro Y ; Yoshimura T ; Tanaka T ; Ishikubo H ; Hiraoka Y ; Takemoto N ; Tanaka T ; Ooguchi Y ; Skehel P ; Aida T ; Takeda J ; Mashimo T BMC Genomics 2018[May]; 19 (1 ): 318 PMID29720086 show ga BACKGROUND: CRISPR/Cas9 enables the targeting of genes in zygotes; however, efficient approaches to create loxP-flanked (floxed) alleles remain elusive. RESULTS: Here, we show that the electroporation of Cas9, two gRNAs, and long single-stranded DNA (lssDNA) into zygotes, termed CLICK (CRISPR with lssDNA inducing conditional knockout alleles), enables the quick generation of floxed alleles in mice and rats. CONCLUSIONS: The high efficiency of CLICK provides homozygous knock-ins in oocytes carrying tissue-specific Cre, which allows the one-step generation of conditional knockouts in founder (F0) mice. |Alleles [MESH] |Animals [MESH] |Base Sequence [MESH] |CRISPR-Cas Systems/genetics [MESH] |Genetic Engineering/*methods [MESH] |Injections [MESH] |Mice [MESH] |Mice, Knockout [MESH]CLICK: one-step generation of conditional knockout mice (epmc).pdf DeepDyve Pubget Overpricing