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10.1107/S2052252516018017

http://scihub22266oqcxt.onion/10.1107/S2052252516018017
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suck abstract from ncbi


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pmid28250941
      IUCrJ 2017 ; 4 (Pt 1 ): 50-64
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  • CADEE: Computer-Aided Directed Evolution of Enzymes #MMPMID28250941
  • Amrein BA ; Steffen-Munsberg F ; Szeler I ; Purg M ; Kulkarni Y ; Kamerlin SC
  • IUCrJ 2017[Jan]; 4 (Pt 1 ): 50-64 PMID28250941 show ga
  • The tremendous interest in enzymes as biocatalysts has led to extensive work in enzyme engineering, as well as associated methodology development. Here, a new framework for computer-aided directed evolution of enzymes (CADEE) is presented which allows a drastic reduction in the time necessary to prepare and analyze in silico semi-automated directed evolution of enzymes. A pedagogical example of the application of CADEE to a real biological system is also presented in order to illustrate the CADEE workflow.
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