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2014 ; 10
(9
): 3969-77
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English Wikipedia
Bovine and equine peritubular and intertubular dentin
#MMPMID24911530
Stock SR
; Deymier-Black AC
; Veis A
; Telser A
; Lux E
; Cai Z
Acta Biomater
2014[Sep]; 10
(9
): 3969-77
PMID24911530
show ga
Dentin contains 1-2?m diameter tubules extending from the pulp cavity to near the
junction with enamel. Peritubular dentin (PTD) borders the tubule lumens and is
surrounded by intertubular dentin (ITD). Differences in PTD and ITD composition
and microstructure remain poorly understood. Here, a (?200nm)(2), 10.1keV
synchrotron X-ray beam maps X-ray fluorescence and X-ray diffraction
simultaneously around tubules in 15-30?m thick bovine and equine specimens.
Increased Ca fluorescence surrounding tubule lumens confirms that PTD is present,
and the relative intensities in PTD and ITD correspond to carbonated apatite
(cAp) volume fraction of ?0.8 in PTD vs. 0.65 assumed for ITD. In the PTD near
the lumen edges, Zn intensity is strongly peaked, corresponding to a Zn content
of ?0.9mgg(-1) for an assumed concentration of ?0.4mgg(-1) for ITD. In the equine
specimen, the Zn K-edge position indicates that Zn(2+) is present, similar to
bovine dentin (Deymier-Black et al., 2013), and the above edge structure is
consistent with spectra from macromolecules related to biomineralization.
Transmission X-ray diffraction shows only cAp, and the 00.2 diffraction peak
(Miller-Bravais indices) width is constant from ITD to the lumen edge. The cAp
00.2 average preferred orientation is axisymmetric (about the tubule axis) in
both bovine and equine dentin, and the axisymmetric preferred orientation
continues from ITD through the PTD to the tubule lumen. These data indicate that
cAp structure does not vary from PTD to ITD.