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10.1016/j.actbio.2014.05.027

http://scihub22266oqcxt.onion/10.1016/j.actbio.2014.05.027
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suck abstract from ncbi


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pmid24911530
      Acta+Biomater 2014 ; 10 (9 ): 3969-77
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  • Bovine and equine peritubular and intertubular dentin #MMPMID24911530
  • Stock SR ; Deymier-Black AC ; Veis A ; Telser A ; Lux E ; Cai Z
  • Acta Biomater 2014[Sep]; 10 (9 ): 3969-77 PMID24911530 show ga
  • Dentin contains 1-2?m diameter tubules extending from the pulp cavity to near the junction with enamel. Peritubular dentin (PTD) borders the tubule lumens and is surrounded by intertubular dentin (ITD). Differences in PTD and ITD composition and microstructure remain poorly understood. Here, a (?200nm)(2), 10.1keV synchrotron X-ray beam maps X-ray fluorescence and X-ray diffraction simultaneously around tubules in 15-30?m thick bovine and equine specimens. Increased Ca fluorescence surrounding tubule lumens confirms that PTD is present, and the relative intensities in PTD and ITD correspond to carbonated apatite (cAp) volume fraction of ?0.8 in PTD vs. 0.65 assumed for ITD. In the PTD near the lumen edges, Zn intensity is strongly peaked, corresponding to a Zn content of ?0.9mgg(-1) for an assumed concentration of ?0.4mgg(-1) for ITD. In the equine specimen, the Zn K-edge position indicates that Zn(2+) is present, similar to bovine dentin (Deymier-Black et al., 2013), and the above edge structure is consistent with spectra from macromolecules related to biomineralization. Transmission X-ray diffraction shows only cAp, and the 00.2 diffraction peak (Miller-Bravais indices) width is constant from ITD to the lumen edge. The cAp 00.2 average preferred orientation is axisymmetric (about the tubule axis) in both bovine and equine dentin, and the axisymmetric preferred orientation continues from ITD through the PTD to the tubule lumen. These data indicate that cAp structure does not vary from PTD to ITD.
  • |Animals [MESH]
  • |Calcium/analysis [MESH]
  • |Cattle [MESH]
  • |Crystallography, X-Ray [MESH]
  • |Dentin/*chemistry/diagnostic imaging [MESH]
  • |Fluorescence [MESH]
  • |Horses [MESH]
  • |Radiography [MESH]
  • |X-Ray Absorption Spectroscopy [MESH]


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