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2015 ; 4
(6
): 995-1003
Nephropedia Template TP
Kim AR
; Olsen JL
; England SJ
; Huang YS
; Fegan KH
; Delgadillo LF
; McGrath KE
; Kingsley PD
; Waugh RE
; Palis J
Stem Cell Reports
2015[Jun]; 4
(6
): 995-1003
PMID26028528
show ga
Red blood cells (RBCs), responsible for oxygen delivery and carbon dioxide
exchange, are essential for our well-being. Alternative RBC sources are needed to
meet the increased demand for RBC transfusions projected to occur as our
population ages. We previously have discovered that erythroblasts derived from
the early mouse embryo can self-renew extensively ex vivo for many months. To
better understand the mechanisms regulating extensive erythroid self-renewal,
global gene expression data sets from self-renewing and differentiating
erythroblasts were analyzed and revealed the differential expression of Bmi-1.
Bmi-1 overexpression conferred extensive self-renewal capacity upon adult
bone-marrow-derived self-renewing erythroblasts, which normally have limited
proliferative potential. Importantly, Bmi-1 transduction did not interfere with
the ability of extensively self-renewing erythroblasts (ESREs) to terminally
mature either in vitro or in vivo. Bmi-1-induced ESREs can serve to generate in
vitro models of erythroid-intrinsic disorders and ultimately may serve as a
source of cultured RBCs for transfusion therapy.
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