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2014 ; 59
(ä): 208-15
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Bioplasmonic calligraphy for multiplexed label-free biodetection
#MMPMID24727607
Tian L
; Tadepalli S
; Park SH
; Liu KK
; Morrissey JJ
; Kharasch ED
; Naik RR
; Singamaneni S
Biosens Bioelectron
2014[Sep]; 59
(ä): 208-15
PMID24727607
show ga
Printable multi-marker biochips that enable simultaneous quantitative detection
of multiple target biomarkers in point-of-care and resource-limited settings are
a holy grail in the field of biodiagnostics. However, preserving the
functionality of biomolecules, which are routinely employed as recognition
elements, during conventional printing approaches remains challenging. In this
article, we introduce a simple yet powerful approach, namely plasmonic
calligraphy, for realizing multiplexed label-free bioassays. Plasmonic
calligraphy involves a regular ballpoint pen filled with biofunctionalized gold
nanorods as plasmonic ink for creating isolated test domains on paper substrates.
Biofriendly plasmonic calligraphy approach serves as a facile method to
miniaturize the test domain size to few mm(2), which significantly improves the
sensitivity of the plasmonic biosensor compared to bioplasmonic paper fabricated
using immersion approach. Furthermore, plasmonic calligraphy also serves as a
simple and efficient means to isolate multiple test domains on a single test
strip, which facilitates multiplexed biodetection and multi-marker biochips.
Plasmonic calligraphy, which can be potentially automated by implementing with a
robotic arm, serves as an alternate path forward to overcome the limitations of
conventional ink-jet printing.