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2011 ; 34
(5
): 225-34
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Biophysical profiling of tumor cell lines
#MMPMID21988886
Coffman F
; Hamid R
; Cohen MC
; Garippa R
; Cohen S
Anal Cell Pathol (Amst)
2011[]; 34
(5
): 225-34
PMID21988886
show ga
Despite significant differences in genetic profiles, cancer cells share common
phenotypic properties, including membrane-associated changes that facilitate
invasion and metastasis. The Corning Epic optical biosensor was used to monitor
dynamic mass rearrangements within and proximal to the cell membrane in tumor
cell lines derived from cancers of the colon, bone, cervix, lung and breast. Data
was collected in real time and required no exogenously added signaling moiety
(signal-free technology). Cell lines displayed unique profiles over the
time-courses: the time-courses all displayed initial signal increases to maximal
values, but the rate of increase to those maxima and the value of those maxima
were distinct for each cell line. The rate of decline following the maxima also
differed among cell lines. There were correlations between the signal maxima and
the observed metastatic behavior of the cells in xenograft experiments; for most
cell types the cells that were more highly metastatic in mice had lower
time-course maxima values, however the reverse was seen in breast cancer cells.
The unique profiles of these cell lines and the correlation of at least one
profile characteristic with metastatic behavior demonstrate the potential utility
of biophysical tumor cell profiling in the study of cancer biology.