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2014 ; 3
(8
): 627-33
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Biological 2-input decoder circuit in human cells
#MMPMID24694115
Guinn M
; Bleris L
ACS Synth Biol
2014[Aug]; 3
(8
): 627-33
PMID24694115
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Decoders are combinational circuits that convert information from n inputs to a
maximum of 2(n) outputs. This operation is of major importance in computing
systems yet it is vastly underexplored in synthetic biology. Here, we present a
synthetic gene network architecture that operates as a biological decoder in
human cells, converting 2 inputs to 4 outputs. As a proof-of-principle, we use
small molecules to emulate the two inputs and fluorescent reporters as the
corresponding four outputs. The experiments are performed using transient
transfections in human kidney embryonic cells and the characterization by
fluorescence microscopy and flow cytometry. We show a clear separation between
the ON and OFF mean fluorescent intensity states. Additionally, we adopt the
integrated mean fluorescence intensity for the characterization of the circuit
and show that this metric is more robust to transfection conditions when compared
to the mean fluorescent intensity. To conclude, we present the first
implementation of a genetic decoder. This combinational system can be valuable
toward engineering higher-order circuits as well as accommodate a multiplexed
interface with endogenous cellular functions.
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