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Bioinformatic prediction of WSSV-host protein-protein interaction
#MMPMID24982879
Sun Z
; Li S
; Li F
; Xiang J
Biomed Res Int
2014[]; 2014
(?): 416543
PMID24982879
show ga
WSSV is one of the most dangerous pathogens in shrimp aquaculture. However, the
molecular mechanism of how WSSV interacts with shrimp is still not very clear. In
the present study, bioinformatic approaches were used to predict interactions
between proteins from WSSV and shrimp. The genome data of WSSV (NC_003225.1) and
the constructed transcriptome data of F. chinensis were used to screen
potentially interacting proteins by searching in protein interaction databases,
including STRING, Reactome, and DIP. Forty-four pairs of proteins were suggested
to have interactions between WSSV and the shrimp. Gene ontology analysis revealed
that 6 pairs of these interacting proteins were classified into "extracellular
region" or "receptor complex" GO-terms. KEGG pathway analysis showed that they
were involved in the "ECM-receptor interaction pathway." In the 6 pairs of
interacting proteins, an envelope protein called "collagen-like protein"
(WSSV-CLP) encoded by an early virus gene "wsv001" in WSSV interacted with 6
deduced proteins from the shrimp, including three integrin alpha (ITGA), two
integrin beta (ITGB), and one syndecan (SDC). Sequence analysis on WSSV-CLP,
ITGA, ITGB, and SDC revealed that they possessed the sequence features for
protein-protein interactions. This study might provide new insights into the
interaction mechanisms between WSSV and shrimp.
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