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Biofilm-associated proteins: news from Acinetobacter
#MMPMID26572057
De Gregorio E
; Del Franco M
; Martinucci M
; Roscetto E
; Zarrilli R
; Di Nocera PP
BMC Genomics
2015[Nov]; 16
(?): 933
PMID26572057
show ga
BACKGROUND: A giant protein called BAP (biofilm-associated protein) plays a role
in biofilm formation and adhesion to host cells in A. baumannii. Most of the
protein is made by arrays of 80-110 aa modules featuring immunoglobulin-like
(Ig-like) motifs. RESULTS: The survey of 541 A. baumannii sequenced strains
belonging to 108 STs (sequence types) revealed that BAP is highly polymorphic,
distinguishable in three main types for changes both in the repetitive and the
COOH region. Analyzing the different STs, we found that 29 % feature type-1, 40 %
type-2 BAP, 11 % type-3 BAP, 20 % lack BAP. The type-3 variant is restricted to
A. baumannii, type-1 and type-2 BAP have been identified also in other species of
the Acinetobacter calcoaceticus-baumannii (ACB) complex. A. calcoaceticus and A.
pittii also encode BAP-like proteins in which Ig-like repeats are replaced by
long tracts of alternating serine and aspartic acid residues. We have identified
in species of the ACB complex two additional proteins, BLP1 and BLP2 (BAP-like
proteins 1 and 2) which feature Ig-like repeats, share with BAP a sequence motif
at the NH2 terminus, and are similarly expressed in stationary growth phase. The
knock-out of either BLP1 or BLP2 genes of the A. baumannii ST1 AYE strain
severely affected biofilm formation, as measured by comparing biofilm biomass and
thickness, and adherence to epithelial cells. BLP1 is missing in the majority of
type-3 BAP strains. BLP2 is largely conserved, but is frequently missing in
BAP-negative cells. CONCLUSIONS: Multiple proteins sharing Ig-like repeats seem
to be involved in biofilm formation. The uneven distribution of the different BAP
types, BLP1, and BLP2 is highly indicative that alternative protein complexes
involved in biofilm formation are assembled in different A. baumannii strains.
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