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2016 ; 31
(5
): 684-692
Nephropedia Template TP
Fan R
; Piou M
; Darling E
; Cormier D
; Sun J
; Wan J
J Biomater Appl
2016[Nov]; 31
(5
): 684-692
PMID27638155
show ga
3D printing of biological architectures that mimic the structural and functional
features of in vivo tissues is of great interest in tissue engineering and the
development of transplantable organ constructs. Printable bio-inks that are
compatible with cellular activities play critical roles in the process of 3D
bio-printing. Although a variety of hydrogels have been used as bio-inks for 3D
bio-printing, they inherit poor mechanical properties and/or the lack of
essential protein components that compromise their performance. Here, a hybrid
Matrigel-agarose hydrogel system has been demonstrated that possesses both
desired rheological properties for bio-printing and biocompatibility for
long-term (11 days) cell culture. The agarose component in the hybrid hydrogel
system enables the maintenance of 3D-printed structures, whereas Matrigel
provides essential microenvironments for cell growth. When human intestinal
epithelial HCT116 cells are encapsulated in the printed Matrigel-agarose
constructs, high cell viability and proper cell spreading morphology are
observed. Given that Matrigel is used extensively for 3D cell culturing, the
developed 3D-printable Matrigel-agarose system will open a new way to construct
Matrigel-based 3D constructs for cell culture and tissue engineering.
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