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10.1021/sb500291r http://scihub22266oqcxt.onion/10.1021/sb500291r C4277767!4277767 !25350266     free     free     free Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\25350266 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       ACS+Synth+Biol 2014 ; 3 (11 ): 832-8 Nephropedia Template TP {{tp|p=25350266 |t=2014. Benchmarking of optical dimerizer systems |pdf=|usr=}}{{25350266 }} gab.com Text Benchmarking of optical dimerizer systems JO: ACS Synth Biol http://www.kidney.de/pget.php?&tw=1&pmid=25350266 #FOAvip Optical dimerizers are a powerful new class of optogenetic tools that allow light-inducible control of protein-protein interactions. Such tools have been useful for regulating cellular pathways and processes with high spatiotemporal resolution in live cells, and a growing number of dimerizer systems are available. As these systems have been characterized by different groups using different methods, it has been difficult for users to compare their properties. Here, we set about to systematically benchmark the properties of four optical dimerizer systems, CRY2/CIB1, TULIPs, phyB/PIF3, and phyB/PIF6. Using a yeast transcriptional assay, we find significant differences in light sensitivity and fold-activation levels between the red light regulated systems but similar responses between the CRY2/CIB and TULIP systems. Further comparison of the ability of the CRY2/CIB1 and TULIP systems to regulate a yeast MAPK signaling pathway also showed similar responses, with slightly less background activity in the dark observed with CRY2/CIB. In the process of developing this work, we also generated an improved blue-light-regulated transcriptional system using CRY2/CIB in yeast. In addition, we demonstrate successful application of the CRY2/CIB dimerizers using a membrane-tethered CRY2, which may allow for better local control of protein interactions. Taken together, this work allows for a better understanding of the capacities of these different dimerization systems and demonstrates new uses of these dimerizers to control signaling and transcription in yeast. Twit Text FOAVip Benchmarking of optical dimerizer systems ????ACS Synth Biol http://www.kidney.de/pget.php?&tw=1&pmid=25350266 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=25350266 #FOAvip English Wikipedia <ref>{{Cite pmid|25350266 }}</ref> Benchmarking of optical dimerizer systems #MMPMID25350266 Pathak GP ; Strickland D ; Vrana JD ; Tucker CL ACS Synth Biol 2014[Nov]; 3 (11 ): 832-8 PMID25350266 show ga Optical dimerizers are a powerful new class of optogenetic tools that allow light-inducible control of protein-protein interactions. Such tools have been useful for regulating cellular pathways and processes with high spatiotemporal resolution in live cells, and a growing number of dimerizer systems are available. As these systems have been characterized by different groups using different methods, it has been difficult for users to compare their properties. Here, we set about to systematically benchmark the properties of four optical dimerizer systems, CRY2/CIB1, TULIPs, phyB/PIF3, and phyB/PIF6. Using a yeast transcriptional assay, we find significant differences in light sensitivity and fold-activation levels between the red light regulated systems but similar responses between the CRY2/CIB and TULIP systems. Further comparison of the ability of the CRY2/CIB1 and TULIP systems to regulate a yeast MAPK signaling pathway also showed similar responses, with slightly less background activity in the dark observed with CRY2/CIB. In the process of developing this work, we also generated an improved blue-light-regulated transcriptional system using CRY2/CIB in yeast. In addition, we demonstrate successful application of the CRY2/CIB dimerizers using a membrane-tethered CRY2, which may allow for better local control of protein interactions. Taken together, this work allows for a better understanding of the capacities of these different dimerization systems and demonstrates new uses of these dimerizers to control signaling and transcription in yeast. |Cryptochromes/chemistry/genetics/*metabolism [MESH] |Dimerization [MESH] |Extracellular Signal-Regulated MAP Kinases [MESH] |Optogenetics/*methods [MESH] |Phytochrome/chemistry/genetics/*metabolism [MESH] |Protein Structure, Tertiary/genetics [MESH] |Signal Transduction/genetics/physiology [MESH] |Transcription, Genetic/*genetics [MESH]Benchmarking of optical dimerizer systems (epmc).pdf DeepDyve Pubget Overpricing