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2018 ; 10
(6
): ä Nephropedia Template TP
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English Wikipedia
Basics of Antibody Phage Display Technology
#MMPMID29890762
Ledsgaard L
; Kilstrup M
; Karatt-Vellatt A
; McCafferty J
; Laustsen AH
Toxins (Basel)
2018[Jun]; 10
(6
): ä PMID29890762
show ga
Antibody discovery has become increasingly important in almost all areas of
modern medicine. Different antibody discovery approaches exist, but one that has
gained increasing interest in the field of toxinology and antivenom research is
phage display technology. In this review, the lifecycle of the M13 phage and the
basics of phage display technology are presented together with important factors
influencing the success rates of phage display experiments. Moreover, the pros
and cons of different antigen display methods and the use of naïve versus
immunized phage display antibody libraries is discussed, and selected examples
from the field of antivenom research are highlighted. This review thus provides
in-depth knowledge on the principles and use of phage display technology with a
special focus on discovery of antibodies that target animal toxins.
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