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2012 ; 889
(ä): 85-103
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Avian models in teratology and developmental toxicology
#MMPMID22669661
Smith SM
; Flentke GR
; Garic A
Methods Mol Biol
2012[]; 889
(ä): 85-103
PMID22669661
show ga
The avian embryo is a long-standing model for developmental biology research. It
also has proven utility for toxicology research both in ovo and in explant
culture. Like mammals, avian embryos have an allantois and their developmental
pathways are highly conserved with those of mammals, thus avian models have
biomedical relevance. Fertile eggs are inexpensive and the embryo develops
rapidly, allowing for high-throughput. The chick genome is sequenced and
significant molecular resources are available for study, including the ability
for genetic manipulation. The absence of a placenta permits the direct study of
an agent's embryotoxic effects. Here, we present protocols for using avian
embryos in toxicology research, including egg husbandry and hatch, toxicant
delivery, and assessment of proliferation, apoptosis, and cardiac structure and
function.