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10.3390/microarrays3040302

http://scihub22266oqcxt.onion/10.3390/microarrays3040302
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suck abstract from ncbi


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pmid27600350
      Microarrays+(Basel) 2014 ; 3 (4 ): 302-21
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  • Assessing Agreement between miRNA Microarray Platforms #MMPMID27600350
  • Bassani NP ; Ambrogi F ; Biganzoli EM
  • Microarrays (Basel) 2014[Dec]; 3 (4 ): 302-21 PMID27600350 show ga
  • Over the last few years, miRNA microarray platforms have provided great insights into the biological mechanisms underlying the onset and development of several diseases. However, only a few studies have evaluated the concordance between different microarray platforms using methods that took into account measurement error in the data. In this work, we propose the use of a modified version of the Bland-Altman plot to assess agreement between microarray platforms. To this aim, two samples, one renal tumor cell line and a pool of 20 different human normal tissues, were profiled using three different miRNA platforms (Affymetrix, Agilent, Illumina) on triplicate arrays. Intra-platform reliability was assessed by calculating pair-wise concordance correlation coefficients (CCC) between technical replicates and overall concordance correlation coefficient (OCCC) with bootstrap percentile confidence intervals, which revealed moderate-to-good repeatability of all platforms for both samples. Modified Bland-Altman analysis revealed good patterns of concordance for Agilent and Illumina, whereas Affymetrix showed poor-to-moderate agreement for both samples considered. The proposed method is useful to assess agreement between array platforms by modifying the original Bland-Altman plot to let it account for measurement error and bias correction and can be used to assess patterns of concordance between other kinds of arrays other than miRNA microarrays.
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