Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\27600350
.jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117 Microarrays+(Basel)
2014 ; 3
(4
): 302-21
Nephropedia Template TP
gab.com Text
Twit Text FOAVip
Twit Text #
English Wikipedia
Assessing Agreement between miRNA Microarray Platforms
#MMPMID27600350
Bassani NP
; Ambrogi F
; Biganzoli EM
Microarrays (Basel)
2014[Dec]; 3
(4
): 302-21
PMID27600350
show ga
Over the last few years, miRNA microarray platforms have provided great insights
into the biological mechanisms underlying the onset and development of several
diseases. However, only a few studies have evaluated the concordance between
different microarray platforms using methods that took into account measurement
error in the data. In this work, we propose the use of a modified version of the
Bland-Altman plot to assess agreement between microarray platforms. To this aim,
two samples, one renal tumor cell line and a pool of 20 different human normal
tissues, were profiled using three different miRNA platforms (Affymetrix,
Agilent, Illumina) on triplicate arrays. Intra-platform reliability was assessed
by calculating pair-wise concordance correlation coefficients (CCC) between
technical replicates and overall concordance correlation coefficient (OCCC) with
bootstrap percentile confidence intervals, which revealed moderate-to-good
repeatability of all platforms for both samples. Modified Bland-Altman analysis
revealed good patterns of concordance for Agilent and Illumina, whereas
Affymetrix showed poor-to-moderate agreement for both samples considered. The
proposed method is useful to assess agreement between array platforms by
modifying the original Bland-Altman plot to let it account for measurement error
and bias correction and can be used to assess patterns of concordance between
other kinds of arrays other than miRNA microarrays.