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2017 ; 6
(3
): ä Nephropedia Template TP
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English Wikipedia
Assays to Monitor Autophagy in Saccharomyces cerevisiae
#MMPMID28703742
Torggler R
; Papinski D
; Kraft C
Cells
2017[Jul]; 6
(3
): ä PMID28703742
show ga
Autophagy is an intracellular process responsible for the degradation and
recycling of cytoplasmic components. It selectively removes harmful cellular
material and enables the cell to survive starvation by mobilizing nutrients via
the bulk degradation of cytoplasmic components. While research over the last
decades has led to the discovery of the key factors involved in autophagy, the
pathway is not yet completely understood. The first studies of autophagy on a
molecular level were conducted in the yeast Saccharomyces cerevisiae. Building up
on these studies, many homologs have been found in higher eukaryotes. Yeast
remains a highly relevant model organism for studying autophagy, with a wide
range of established methods to elucidate the molecular details of the autophagy
pathway. In this review, we provide an overview of methods to study both
selective and bulk autophagy, including intermediate steps in the yeast
Saccharomyces cerevisiae. We compare different assays, discuss their advantages
and limitations and list potential applications.