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2015 ; 6
(2
): 409-18
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Assaying Visual Memory in the Desert Locust
#MMPMID26463192
Dillen S
; Chen Z
; Broeck JV
Insects
2015[Apr]; 6
(2
): 409-18
PMID26463192
show ga
The involvement of associative learning cues has been demonstrated in several
stages of feeding and food selection. Short neuropeptide F (sNPF), an insect
neuropeptide whose effects on feeding behavior have previously been well
established, may be one of the factors bridging feeding and learning behavior.
Recently, it was shown in Drosophila melanogaster that the targeted reduction of
Drome-sNPF transcript levels significantly reduced sugar-rewarded olfactory
memory. While Drosophila mainly relies on olfactory perception in its food
searching behavior, locust foraging behavior is likely to be more visually
orientated. Furthermore, a feeding-dependent regulation of Schgr-sNPF transcript
levels has previously been observed in the optic lobes of the locust brain,
suggesting a possible involvement in visual perception of food and visual
associative memory in this insect species. In this study, we describe the
development of a robust and reproducible assay allowing visual associative memory
to be studied in the desert locust, Schistocerca gregaria. Furthermore, we
performed an exploratory series of experiments, studying the role of Schgr-sNPF
in this complex process.