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2017 ; 356
(6334
): 194-197
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Architecture of a transcribing-translating expressome
#MMPMID28408604
Kohler R
; Mooney RA
; Mills DJ
; Landick R
; Cramer P
Science
2017[Apr]; 356
(6334
): 194-197
PMID28408604
show ga
DNA transcription is functionally coupled to messenger RNA (mRNA) translation in
bacteria, but how this is achieved remains unclear. Here we show that RNA
polymerase (RNAP) and the ribosome of Escherichia coli can form a defined
transcribing and translating "expressome" complex. The cryo-electron microscopic
structure of the expressome reveals continuous protection of ~30 nucleotides of
mRNA extending from the RNAP active center to the ribosome decoding center. The
RNAP-ribosome interface includes the RNAP subunit ? carboxyl-terminal domain,
which is required for RNAP-ribosome interaction in vitro and for pronounced cell
growth defects upon translation inhibition in vivo, consistent with its function
in transcription-translation coupling. The expressome structure can only form
during transcription elongation and explains how translation can prevent
transcriptional pausing, backtracking, and termination.
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