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10.1021/ac202335u http://scihub22266oqcxt.onion/10.1021/ac202335u C4042682!4042682 !22017663     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=22017663 &cmd=llinks): Failed to open stream: HTTP request failed! HTTP/1.1 429 Too Many Requests in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 215 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\22017663 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Anal+Chem 2011 ; 83 (23 ): 9138-45 Nephropedia Template TP {{tp|p=22017663 |t=2011. Aptamer-functionalized microgel particles for protein detection |pdf=|usr=}}{{22017663 }} gab.com Text Aptamer-functionalized microgel particles for protein detection JO: Anal Chem http://www.kidney.de/pget.php?&tw=1&pmid=22017663 #FOAvip Highly sensitive and multiplexed detection of clinically relevant proteins in biologically complex samples is crucial for the advancement of clinical proteomics. In recent years, aptamers have emerged as useful tools for protein analysis due to their specificity and affinity for protein targets as well as their compatibility with particle-based detection systems. In this study, we demonstrate the highly sensitive detection of human ?-thrombin on encoded hydrogel microparticles functionalized with an aptamer capture sequence. We use static imaging and microfluidic flow-through analysis techniques to evaluate the detection capabilities of the microgels in sandwich-assay formats that utilize both aptamers and antibodies for the reporting of target-binding events. Buffers and reagent concentrations were optimized to provide maximum reaction efficiency while still maintaining an assay with a simple workflow that can be easily adapted to the multiplexed detection of other clinically relevant proteins. The three-dimensional, nonfouling hydrogel immobilization scaffold used in this work provides three logs of dynamic range, with a limit of detection of 4 pM using a single aptamer capture species and without the need for spacers or signal amplification. Twit Text FOAVip Aptamer-functionalized microgel particles for protein detection ????Anal Chem http://www.kidney.de/pget.php?&tw=1&pmid=22017663 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=22017663 #FOAvip English Wikipedia <ref>{{Cite pmid|22017663 }}</ref> Aptamer-functionalized microgel particles for protein detection #MMPMID22017663 Srinivas RL ; Chapin SC ; Doyle PS Anal Chem 2011[Dec]; 83 (23 ): 9138-45 PMID22017663 show ga Highly sensitive and multiplexed detection of clinically relevant proteins in biologically complex samples is crucial for the advancement of clinical proteomics. In recent years, aptamers have emerged as useful tools for protein analysis due to their specificity and affinity for protein targets as well as their compatibility with particle-based detection systems. In this study, we demonstrate the highly sensitive detection of human ?-thrombin on encoded hydrogel microparticles functionalized with an aptamer capture sequence. We use static imaging and microfluidic flow-through analysis techniques to evaluate the detection capabilities of the microgels in sandwich-assay formats that utilize both aptamers and antibodies for the reporting of target-binding events. Buffers and reagent concentrations were optimized to provide maximum reaction efficiency while still maintaining an assay with a simple workflow that can be easily adapted to the multiplexed detection of other clinically relevant proteins. The three-dimensional, nonfouling hydrogel immobilization scaffold used in this work provides three logs of dynamic range, with a limit of detection of 4 pM using a single aptamer capture species and without the need for spacers or signal amplification. |Aptamers, Nucleotide/*chemistry [MESH] |Buffers [MESH] |Humans [MESH] |Hydrogels/*chemistry [MESH] |Osmolar Concentration [MESH]Aptamer-functionalized microgel particles for protein detection (epmc).pdf DeepDyve Pubget Overpricing