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10.1194/jlr.D060764 http://scihub22266oqcxt.onion/10.1194/jlr.D060764 C4548786!4548786 !26142958     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=26142958 &cmd=llinks): Failed to open stream: HTTP request failed! HTTP/1.1 429 Too Many Requests in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 215 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\26142958 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       J+Lipid+Res 2015 ; 56 (9 ): 1821-35 Nephropedia Template TP {{tp|p=26142958 |t=2015. Apprehending ganglioside diversity: a comprehensive methodological approach |pdf=|usr=}}{{26142958 }} gab.com Text Apprehending ganglioside diversity: a comprehensive methodological approach JO: J Lipid Res http://www.kidney.de/pget.php?&tw=1&pmid=26142958 #FOAvip Gangliosides (GGs) make a wide family of glycosphingolipids ubiquitously expressed in mammalian tissues and particularly abundant in the brain and nervous system. They exhibit a huge diversity due to structural variations in both their oligosaccharidic chain and ceramide moiety, which represent a real analytical challenge. Since their discovery in the 1940s, methods have persistently improved until the emergence of LC/MS, which offers a high level of specificity and sensitivity and is suitable with high-throughput profiling studies. We describe here a comprehensive approach relying on various techniques and aiming at fully characterizing GGs in biological samples. First, total GG content was determined by a biochemical assay. Second, GG class composition was assessed by high-performance thin-layer chromatography followed by colorimetric revelation. Then, ceramide types of GG classes were identified, and their relative quantification was performed thanks to the development of a powerful and reliable LC/MS method. Finally, ceramides were structurally characterized, and minor and less common GG classes were identified using high-resolution MS. These methods were applied to the rat retina to provide an exhaustive description of its GG composition, giving the base for a better understanding of the precise roles of GGs in this tissue. Twit Text FOAVip Apprehending ganglioside diversity: a comprehensive methodological approach ????J Lipid Res http://www.kidney.de/pget.php?&tw=1&pmid=26142958 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=26142958 #FOAvip English Wikipedia <ref>{{Cite pmid|26142958 }}</ref> Apprehending ganglioside diversity: a comprehensive methodological approach #MMPMID26142958 Masson EA ; Sibille E ; Martine L ; Chaux-Picquet F ; Bretillon L ; Berdeaux O J Lipid Res 2015[Sep]; 56 (9 ): 1821-35 PMID26142958 show ga Gangliosides (GGs) make a wide family of glycosphingolipids ubiquitously expressed in mammalian tissues and particularly abundant in the brain and nervous system. They exhibit a huge diversity due to structural variations in both their oligosaccharidic chain and ceramide moiety, which represent a real analytical challenge. Since their discovery in the 1940s, methods have persistently improved until the emergence of LC/MS, which offers a high level of specificity and sensitivity and is suitable with high-throughput profiling studies. We describe here a comprehensive approach relying on various techniques and aiming at fully characterizing GGs in biological samples. First, total GG content was determined by a biochemical assay. Second, GG class composition was assessed by high-performance thin-layer chromatography followed by colorimetric revelation. Then, ceramide types of GG classes were identified, and their relative quantification was performed thanks to the development of a powerful and reliable LC/MS method. Finally, ceramides were structurally characterized, and minor and less common GG classes were identified using high-resolution MS. These methods were applied to the rat retina to provide an exhaustive description of its GG composition, giving the base for a better understanding of the precise roles of GGs in this tissue. |Animals [MESH] |Brain Chemistry [MESH] |Brain/*metabolism [MESH] |Ceramides/chemistry/*isolation & purification/metabolism [MESH] |Chromatography, Liquid [MESH] |Gangliosides/chemistry/*isolation & purification/metabolism [MESH] |Glycosphingolipids/*isolation & purification/metabolism [MESH] |Nervous System/chemistry/metabolism [MESH] |Rats [MESH] |Retina/chemistry/metabolism [MESH]Apprehending ganglioside diversity: a comprehensive methodological app(epmc).pdf DeepDyve Pubget Overpricing