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Applications of CRISPR Genome Engineering in Cell Biology
#MMPMID27599850
Wang F
; Qi LS
Trends Cell Biol
2016[Nov]; 26
(11
): 875-888
PMID27599850
show ga
Recent advances in genome engineering are starting a revolution in biological
research and translational applications. The clustered regularly interspaced
short palindromic repeats (CRISPR)-associated RNA-guided endonuclease CRISPR
associated protein 9 (Cas9) and its variants enable diverse manipulations of
genome function. In this review, we describe the development of Cas9 tools for a
variety of applications in cell biology research, including the study of
functional genomics, the creation of transgenic animal models, and genomic
imaging. Novel genome engineering methods offer a new avenue to understand the
causality between the genome and phenotype, thus promising a fuller understanding
of cell biology.
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