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2016 ; 25
(5
): 1032-57
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Application of multivariate statistical techniques in microbial ecology
#MMPMID26786791
Paliy O
; Shankar V
Mol Ecol
2016[Mar]; 25
(5
): 1032-57
PMID26786791
show ga
Recent advances in high-throughput methods of molecular analyses have led to an
explosion of studies generating large-scale ecological data sets. In particular,
noticeable effect has been attained in the field of microbial ecology, where new
experimental approaches provided in-depth assessments of the composition,
functions and dynamic changes of complex microbial communities. Because even a
single high-throughput experiment produces large amount of data, powerful
statistical techniques of multivariate analysis are well suited to analyse and
interpret these data sets. Many different multivariate techniques are available,
and often it is not clear which method should be applied to a particular data
set. In this review, we describe and compare the most widely used multivariate
statistical techniques including exploratory, interpretive and discriminatory
procedures. We consider several important limitations and assumptions of these
methods, and we present examples of how these approaches have been utilized in
recent studies to provide insight into the ecology of the microbial world.
Finally, we offer suggestions for the selection of appropriate methods based on
the research question and data set structure.