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2015 ; 95
(4
): 432-52
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Application of GFP imaging in cancer
#MMPMID25686095
Hoffman RM
Lab Invest
2015[Apr]; 95
(4
): 432-52
PMID25686095
show ga
Multicolored proteins have allowed the color-coding of cancer cells growing in
vivo and enabled the distinction of host from tumor with single-cell resolution.
Non-invasive imaging with fluorescent proteins enabled the dynamics of metastatic
cancer to be followed in real time in individual animals. Non-invasive imaging of
cancer cells expressing fluorescent proteins has allowed the real-time
determination of efficacy of candidate antitumor and antimetastatic agents in
mouse models. The use of fluorescent proteins to differentially label cancer
cells in the nucleus and cytoplasm can visualize the nuclear-cytoplasmic dynamics
of cancer cells in vivo including: mitosis, apoptosis, cell-cycle position, and
differential behavior of nucleus and cytoplasm that occurs during cancer-cell
deformation and extravasation. Recent applications of the technology described
here include linking fluorescent proteins with cell-cycle-specific proteins such
that the cells change color from red to green as they transit from G1 to S
phases. With the macro- and micro-imaging technologies described here,
essentially any in vivo process can be imaged, giving rise to the new field of in
vivo cell biology using fluorescent proteins.
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