Application of CRISPR/Cas9 for biomedical discoveries
#MMPMID26137216
Riordan SM
; Heruth DP
; Zhang LQ
; Ye SQ
Cell Biosci
2015[]; 5
(?): 33
PMID26137216
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The Clustered Regions of Interspersed Palindromic Repeats-Cas9 (CRISPR/Cas9), a
viral defense system found in bacteria and archaea, has emerged as a tour de
force genome editing tool. The CRISPR/Cas9 system is much easier to customize and
optimize because the site selection for DNA cleavage is guided by a short
sequence of RNA rather than an engineered protein as in the systems of zinc
finger nucleases (ZFN), transcription activator-like effector nucleases (TALEN),
and meganucleases. Although it still suffers from some off-target effects, the
CRISPR/Cas9 system has been broadly and successfully applied for biomedical
discoveries in a number of areas. In this review, we present a brief history and
development of the CRISPR system and focus on the application of this genome
editing technology for biomedical discoveries. We then present concise concluding
remarks and future directions for this fast moving field.
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