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2017 ; 8
(12
): 19834-19842
Nephropedia Template TP
Meng S
; Zhu Y
; Li JF
; Wang X
; Liang Z
; Li SQ
; Xu X
; Chen H
; Liu B
; Zheng XY
; Xie LP
Oncotarget
2017[Mar]; 8
(12
): 19834-19842
PMID28423637
show ga
Apigenin, a natural flavonoid found in vegetables and fruits, has antitumor
activity in several cancer types. The present study evaluated the effects and
mechanism of action of apigenin in renal cell carcinoma (RCC) cells. We found
that apigenin suppressed ACHN, 786-0, and Caki-1 RCC cell proliferation in a
dose- and time-dependent manner. A comet assay suggested that apigenin caused DNA
damage in ACHN cells, especially at higher doses, and induced G2/M phase cell
cycle arrest through ATM signal modulation. Small interfering RNA
(siRNA)-mediated p53 knockdown showed that apigenin-induced apoptosis was likely
p53 dependent. Apigenin anti-proliferative effects were confirmed in an ACHN cell
xenograft mouse model. Apigenin treatment reduced tumor growth and volume in
vivo, and immunohistochemical staining revealed lower Ki-67 indices in tumors
derived from apigenin-treated mice. These findings suggest that apigenin exposure
induces DNA damage, G2/M phase cell cycle arrest, p53 accumulation and apoptosis,
which collectively suppress ACHN RCC cell proliferation in vitro and in vivo.
Given its antitumor effects and low in vivo toxicity, apigenin is a highly
promising agent for treatment of RCC.
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