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10.1038/ncomms14473 http://scihub22266oqcxt.onion/10.1038/ncomms14473 C5321729!5321729 !28211541     free     free     free Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\28211541 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Nat+Commun 2017 ; 8 (ä): 14473 Nephropedia Template TP {{tp|p=28211541 |t=2017. Antibody-controlled actuation of DNA-based molecular circuits |pdf=|usr=}}{{28211541 }} gab.com Text Antibody-controlled actuation of DNA-based molecular circuits JO: Nat Commun http://www.kidney.de/pget.php?&tw=1&pmid=28211541 #FOAvip DNA-based molecular circuits allow autonomous signal processing, but their actuation has relied mostly on RNA/DNA-based inputs, limiting their application in synthetic biology, biomedicine and molecular diagnostics. Here we introduce a generic method to translate the presence of an antibody into a unique DNA strand, enabling the use of antibodies as specific inputs for DNA-based molecular computing. Our approach, antibody-templated strand exchange (ATSE), uses the characteristic bivalent architecture of antibodies to promote DNA-strand exchange reactions both thermodynamically and kinetically. Detailed characterization of the ATSE reaction allowed the establishment of a comprehensive model that describes the kinetics and thermodynamics of ATSE as a function of toehold length, antibody-epitope affinity and concentration. ATSE enables the introduction of complex signal processing in antibody-based diagnostics, as demonstrated here by constructing molecular circuits for multiplex antibody detection, integration of multiple antibody inputs using logic gates and actuation of enzymes and DNAzymes for signal amplification. Twit Text FOAVip Antibody-controlled actuation of DNA-based molecular circuits ????Nat Commun http://www.kidney.de/pget.php?&tw=1&pmid=28211541 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=28211541 #FOAvip English Wikipedia <ref>{{Cite pmid|28211541 }}</ref> Antibody-controlled actuation of DNA-based molecular circuits #MMPMID28211541 Engelen W ; Meijer LH ; Somers B ; de Greef TF ; Merkx M Nat Commun 2017[Feb]; 8 (ä): 14473 PMID28211541 show ga DNA-based molecular circuits allow autonomous signal processing, but their actuation has relied mostly on RNA/DNA-based inputs, limiting their application in synthetic biology, biomedicine and molecular diagnostics. Here we introduce a generic method to translate the presence of an antibody into a unique DNA strand, enabling the use of antibodies as specific inputs for DNA-based molecular computing. Our approach, antibody-templated strand exchange (ATSE), uses the characteristic bivalent architecture of antibodies to promote DNA-strand exchange reactions both thermodynamically and kinetically. Detailed characterization of the ATSE reaction allowed the establishment of a comprehensive model that describes the kinetics and thermodynamics of ATSE as a function of toehold length, antibody-epitope affinity and concentration. ATSE enables the introduction of complex signal processing in antibody-based diagnostics, as demonstrated here by constructing molecular circuits for multiplex antibody detection, integration of multiple antibody inputs using logic gates and actuation of enzymes and DNAzymes for signal amplification. |Antibodies/*metabolism [MESH] |DNA, Catalytic/metabolism [MESH] |DNA/*metabolism [MESH] |Kinetics [MESH] |Least-Squares Analysis [MESH] |Logic [MESH] |Models, Biological [MESH]Antibody-controlled actuation of DNA-based molecular circuits (epmc).pdf DeepDyve Pubget Overpricing