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10.1105/tpc.15.00589 http://scihub22266oqcxt.onion/10.1105/tpc.15.00589 C4815043!4815043 !26342015     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=26342015 &cmd=llinks): Failed to open stream: HTTP request failed! 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Anthocyanin Vacuolar Inclusions Form by a Microautophagy Mechanism |pdf=|usr=}}{{26342015 }} gab.com Text Anthocyanin Vacuolar Inclusions Form by a Microautophagy Mechanism JO: Plant Cell http://www.kidney.de/pget.php?&tw=1&pmid=26342015 #FOAvip Anthocyanins are flavonoid pigments synthesized in the cytoplasm and stored inside vacuoles. Many plant species accumulate densely packed, 3- to 10-?m diameter anthocyanin deposits called anthocyanin vacuolar inclusions (AVIs). Despite their conspicuousness and importance in organ coloration, the origin and nature of AVIs have remained controversial for decades. We analyzed AVI formation in cotyledons of different Arabidopsis thaliana genotypes grown under anthocyanin inductive conditions and in purple petals of lisianthus (Eustoma grandiorum). We found that cytoplasmic anthocyanin aggregates in close contact with the vacuolar surface are directly engulfed by the vacuolar membrane in a process reminiscent of microautophagy. The engulfed anthocyanin aggregates are surrounded by a single membrane derived from the tonoplast and eventually become free in the vacuolar lumen like an autophagic body. Neither endosomal/prevacuolar trafficking nor the autophagy ATG5 protein is involved in the formation of AVIs. In Arabidopsis, formation of AVIs is promoted by both an increase in cyanidin 3-O-glucoside derivatives and by depletion of the glutathione S-transferase TT19. We hypothesize that this novel microautophagy mechanism also mediates the transport of other flavonoid aggregates into the vacuole. Twit Text FOAVip Anthocyanin Vacuolar Inclusions Form by a Microautophagy Mechanism ????Plant Cell http://www.kidney.de/pget.php?&tw=1&pmid=26342015 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=26342015 #FOAvip English Wikipedia <ref>{{Cite pmid|26342015 }}</ref> Anthocyanin Vacuolar Inclusions Form by a Microautophagy Mechanism #MMPMID26342015 Chanoca A ; Kovinich N ; Burkel B ; Stecha S ; Bohorquez-Restrepo A ; Ueda T ; Eliceiri KW ; Grotewold E ; Otegui MS Plant Cell 2015[Sep]; 27 (9 ): 2545-59 PMID26342015 show ga Anthocyanins are flavonoid pigments synthesized in the cytoplasm and stored inside vacuoles. Many plant species accumulate densely packed, 3- to 10-?m diameter anthocyanin deposits called anthocyanin vacuolar inclusions (AVIs). Despite their conspicuousness and importance in organ coloration, the origin and nature of AVIs have remained controversial for decades. We analyzed AVI formation in cotyledons of different Arabidopsis thaliana genotypes grown under anthocyanin inductive conditions and in purple petals of lisianthus (Eustoma grandiorum). We found that cytoplasmic anthocyanin aggregates in close contact with the vacuolar surface are directly engulfed by the vacuolar membrane in a process reminiscent of microautophagy. The engulfed anthocyanin aggregates are surrounded by a single membrane derived from the tonoplast and eventually become free in the vacuolar lumen like an autophagic body. Neither endosomal/prevacuolar trafficking nor the autophagy ATG5 protein is involved in the formation of AVIs. In Arabidopsis, formation of AVIs is promoted by both an increase in cyanidin 3-O-glucoside derivatives and by depletion of the glutathione S-transferase TT19. We hypothesize that this novel microautophagy mechanism also mediates the transport of other flavonoid aggregates into the vacuole. |Anthocyanins/*metabolism [MESH] |Arabidopsis Proteins/genetics/metabolism [MESH] |Arabidopsis/*cytology/genetics/metabolism [MESH] |Autophagy/*physiology [MESH] |Biological Transport [MESH] |Cytoplasm/metabolism [MESH] |Endoplasmic Reticulum/metabolism [MESH] |Fluorescence [MESH] |Gentianaceae/*cytology/metabolism [MESH] |Glucosides/metabolism [MESH] |Glutathione Transferase/genetics/metabolism [MESH] |Intracellular Membranes/metabolism [MESH] |Mutation [MESH] |Plant Cells/metabolism [MESH] |Plants, Genetically Modified [MESH] |Seedlings/cytology/genetics [MESH]Anthocyanin Vacuolar Inclusions Form by a Microautophagy Mechanism (epmc).pdf DeepDyve Pubget Overpricing