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2018 ; 1672
(ä): 43-61
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Analyzing Genome Rearrangements in Saccharomyces cerevisiae
#MMPMID29043616
Srivatsan A
; Putnam CD
; Kolodner RD
Methods Mol Biol
2018[]; 1672
(ä): 43-61
PMID29043616
show ga
Genome rearrangements underlie different human diseases including many cancers.
Determining the rates at which genome rearrangements arise and isolating unique,
independent genome rearrangements is critical to understanding the genes and
pathways that prevent or promote genome rearrangements. Here, we describe
quantitative S. cerevisiae genetic assays for measuring the rates of accumulating
genome rearrangements including deletions, translocations, and broken chromosomes
healed by de novo telomere addition that result in the deletion of two
counter-selectable genes, CAN1 and URA3, placed in the nonessential regions of
the S. cerevisiae genome. The assays also allow for the isolation of individual
genome rearrangements for structural studies, and a method for analyzing genome
rearrangements by next-generation DNA sequencing is provided.