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2017 ; 17
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Analytical Protein Microarrays: Advancements Towards Clinical Applications
#MMPMID28146048
Sauer U
Sensors (Basel)
2017[Jan]; 17
(2
): ä PMID28146048
show ga
Protein microarrays represent a powerful technology with the potential to serve
as tools for the detection of a broad range of analytes in numerous applications
such as diagnostics, drug development, food safety, and environmental monitoring.
Key features of analytical protein microarrays include high throughput and
relatively low costs due to minimal reagent consumption, multiplexing, fast
kinetics and hence measurements, and the possibility of functional integration.
So far, especially fundamental studies in molecular and cell biology have been
conducted using protein microarrays, while the potential for clinical, notably
point-of-care applications is not yet fully utilized. The question arises what
features have to be implemented and what improvements have to be made in order to
fully exploit the technology. In the past we have identified various obstacles
that have to be overcome in order to promote protein microarray technology in the
diagnostic field. Issues that need significant improvement to make the technology
more attractive for the diagnostic market are for instance: too low sensitivity
and deficiency in reproducibility, inadequate analysis time, lack of high-quality
antibodies and validated reagents, lack of automation and portable instruments,
and cost of instruments necessary for chip production and read-out. The scope of
the paper at hand is to review approaches to solve these problems.