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2013 ; 36
(1-2
): 5-17
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Advanced methods in fluorescence microscopy
#MMPMID23271142
Fritzky L
; Lagunoff D
Anal Cell Pathol (Amst)
2013[]; 36
(1-2
): 5-17
PMID23271142
show ga
It requires a good deal of will power to resist hyperbole in considering the
advances that have been achieved in fluorescence microscopy in the last 25 years.
Our effort has been to survey the modalities of microscopic fluorescence imaging
available to cell biologists and perhaps useful for diagnostic pathologists. The
gamut extends from established confocal laser scanning through multiphoton and
TIRF to the emerging technologies of super-resolution microscopy that breech the
Abbé limit of resolution. Also considered are the recent innovations in
structured and light sheet illumination, the use of FRET and molecular beacons
that exploit specific characteristics of designer fluorescent proteins,
fluorescence speckles, and second harmonic generation for native anisometric
structures like collagen, microtubules and sarcomeres.